Marine collagen and elastin peptides
COLLACTIVE™ is composed of marine collagen and elastin peptides in the same ratio as in the dermis.
COLLACTIVE™ combines the two main constituents of the extracellular matrix and has an anti-wrinkle synergic action: elastin fibres give the dermis suppleness that completes collagen fibres tensile strength.
COLLACTIVE™ polypeptides have a low molecular weight, making it soluble in aqueous phase and fully digestible. COLLACTIVE™ is ideal for nutricosmetics.
COLLACTIVE™ REINFORCES SKIN HYDRATION
The moisturizing effect of COLLACTIVE™ is supported by a clinical study involving 43 women over
40 years old. The results show an increase of the entire epidermis hydration (+8%) after oral
administration of COLLACTIVE™ for 3 months.
• COLLACTIVE™ HELPS MATURE SKIN BUILD LIFT AND FIRMING CUSHION
COLLACTIVE™ collagen and elastin polypeptides stimulate the biosynthesis of dermis main constituents to lift and tone slacking areas.
COLLACTIVE™ minimizes apparent lines and wrinkles as per the results of the clinical study (see graph).
On D84, the skin was exposed to cold temperatures, which explain the weaker anti-wrinkle effect at this period. The results highlight the protective effect of COLLACTIVE™ against the harsh conditions in winter.
While life is increasingly stressful, time and serenity are becoming a priority among
consumers and health, beauty and wellbeing tend to be perceived as a whole. There is an
increasing crossover between the idea of inner and outer wellbeing, inner and outer beauty. In
such a context, COPALIS has developed a natural oral cosmetic active that meets new market
demand for nutricosmetics.
- INTRODUCTION -
Skin characteristics are known to be affected by endogenous and environmental factors, including
aging, exposure to sunlight, chemicals, and mechanical damage. To maintain and improve skin
condition, a wide variety of skin-care products are on the market. However, many skin problems
originate from endogenous sources and food intake is frequently mentioned as influencing skin
condition. Part of our knowledge of the relation between nutrients and skin comes from the incidence
of skin problems as a result of nutritional deficiencies. But it has also been shown that the
optimization of the diet improve skin condition[1, 2, 3].
A study illustrated that skin wrinkling in a sun-exposed site in older people of various ethnic
backgrounds is influenced by the types of foods consumed: 32% of the variance was predicted by
food intake. Oils, fish, vegetables and cereals appeared to be protective
- Being the most abundant protein of the human body, collagen supplementation has been widely
studied. It has been reported that the oral ingestion of collagen peptides affects various functions of
the body. For example, lower bone mineral density in protein malnutrition and joint disease are
improved by ingestion of collagen peptides[4,5]. It has also been reported that hair thickness increased
after prolonged ingestion of collagen peptides and that nail disorders, such as brittle nails, were
improved by collagen peptides intake.
- In the dermis collagen peptides supplementation has been widely studied. Matsuda et al. first
showed that the water absorption ability of the skin stratum corneum of 20 volunteers increased after
the daily ingestion of 10g of collagen peptides for 60 days. They then showed that collagen peptide
supplementation for 62 days induces the increase of fibroblasts density and the increase of collagen
fibrils size the density of fibroblasts, and diameter and density of collagen fibrils in the dermis: the
ingestion of collagen peptides improves the mechanical strength of the skin in a collagen-specific
manner. Taken together, these results suggest that the daily ingestion of collagen peptide improves
the function of both the epidermis and the dermis.
- The effects of the daily ingestion of a collagen hydrolysate mixture (including 5g of type I fish
collagen hydrolysate) on the skin properties of 25 women with dry and rough skin were also studied
by Matsumoto et al.. They observed their skin properties before and after 6 weeks of ingestion. The
following improvements were noted:
o increase of the moisture of the face cheeks, the forearms and the back of the neck
o improvement of the viscoelastic properties (pliability and elasticity)
o skin smoothness, skin wrinkles and skin roughness values improvement (on the cheeks area)
- CHARACTERISATION OF COLLACTIVE™ MOLECULES -
COLLACTIVE™ is composed of marine collagen and elastin peptides in the same ratio as in the
Collagen peptides stem from the hydrolysis of a type 1 collagen, characterised by its triple helix
structure with two identical polypeptide chains (α1(1)) and a third that is genetically different (α 2(1)).
Each of the chains is composed of a sequence of amino acids where the sub-unit Glycine-X-Y is
repeated. Glycine therefore represents 35% of all amino acids. Proline and hydroxyproline are also
present at levels of respectively 13% and 8% of the total amino acids.
COLLACTIVE™ collagen is very similar to human skin collagen. Indeed the dermis predominantly
contains type I collagen (85 to 90%).
Elastin is a macropolymeric protein. Highly insoluble and extensively cross-linked, mature elastin is
formed from tropoelastin, its soluble precursor. Tropoelastin is an <70-kDa protein consisting of an
alternance of hydrophobic regions, responsible for elasticity, and cross-linking domains. Additionally,
it ends with a hydrophilic carboxy-terminal sequence containing its only two cysteine residues.
Collagen and elastin fibers within the skin (figure 2)
Collagen and elastin are abundant fibrous proteins in the skin. Many rodlike collagen molecules are
cross-linked together in the extracellular space to form collagen fibrils that have the tensile strength of
steel. The striping on the collagen fibril is caused by the regular repeating arrangement of the
collagen molecules within the fibril. Elastin polypeptide chains are cross-linked together to form
rubberlike, elastic fibers. Each elastin molecule uncoils into a more extended conformation when the
fibre is stretched and will recoil spontaneously as soon as the stretching force is relaxed.
Elastin fibres give the dermis suppleness that completes collagen fibres tensile strength.
In the dermis, collagen fibres form 70% of the total proteins and are the main constituents of the extracellular matrix along with elastin fibres. Degradation of these two types of fibres is responsible for the appearance of wrinkles and skin ageing.
- HIGH DIGESTIBILITY OF COLLACTIVE™ PEPTIDES -
Hydrolysed collagen and elastin of COLLACTIVE™ can easily be digested due to the peptide nature
of these molecules. Published research has shown that collagen, in its hydrolysed form, can be easily
absorbed. This property makes COLLACTIVE™ effective and beneficial at low doses in dietary
Several studies[11,12] have shown that a substantial increase of collagen specific amino acids in serum
is observed after subsequent oral administration of hydrolysed collagen. Iwai et al. identified a small
peptide (Pro-Hyp) in the serum and plasma of healthy human volunteers who ingested collagen
These results show that the oral administration of COLLACTIVE™ offers a useful source of amino
acids for collagen and elastin biosynthesis.
- COLLACTIVE™ PEPTIDES ANTI-AGEING PROPERTIES -
The tolerance, the anti-wrinkle and the moisturizing properties of the daily ingestion of
COLLACTIVE™ were assessed through a simple insu and parallel group clinical study.
MATERIAL & METHODS
COLLACTIVE™ was put in capsules for its oral administration.
Placebo capsules contained maltodextrin.
A simple insu / parallel group study was performed on 43 healthy volunteers. Inclusion criteria were:
- Sex: female
- Age: between 40 and 55 years old
- Subjects with wrinkles on crow’s-feet
Volunteers were not allowed to use any anti-wrinkle product on the measurement skin area (the use
of this kind of product less than a month before the trial starting date was an exclusion criteria as
Two grams per day of either COLLACTIVE™ or placebo were ingested with a glass of water for 84
days. The subjects’ skin conditions were measured at day 0, day 28 and day 84.
Measurements were done on the forearm and the face.
The anti wrinkles effect was evaluated by the measurement of the variation of different parameters of
the cutaneous relief with Skin Image Analyser® and with Quantirides® software:
1. furrows of cutaneous microrelief (number and depth)
2. medium wrinkles (number and depth)
3. deep wrinkles (number and depth)
The cutaneous hydration rate was assessed using Hydrascan® and Corneometer®.
The cutaneous tolerance was evaluated through a clinical examination and volunteers were asked to
fill a questionnaire to know their subjective evaluation.